Biomethanol Conversion from Sugar Beet Pulp with Pectin Methyl Esterase

Abstract :-
Conversion of renewable biomethanol was studied from sugar beet pulp with pectin methyl esterases (PMEs). An analytical method for methanol and PME activity was developed based on potassium permanganate assay. Results showed that this method was sensitive, accurate and stable. Two PMEs, natural and mutated, were then produced and characterized. The kinetics parameters of Michaelis-Menten model and thermal deactivation model were determined. Continue reading Biomethanol Conversion from Sugar Beet Pulp with Pectin Methyl Esterase

Automation Model For pH and Conductivity Adjustment for a Cation Exchange Column Eluate Buffer

Abstract :-
A comprehensive model for calculating pH and conductivity values for a buffer solution added to the eluate of a cation exchange column at Bayer Healthcare has been developed. Bayer Healthcare’s Berkeley facility manufacturess Kogenate – a drug for Hemophilia. This project was carried out at one of the intermediate purification steps of Kogenate, where the pH and conductivity of a buffer solution added to a chromatographic column’s eluate has to be critically adjusted. Currently this process is done manually and requires two operators. In order to reduce the manual labor as well as improve accuracy, an automation model was developed using feedback control and Bayer’s pH and conductivity predicting empirical correlations. Continue reading Automation Model For pH and Conductivity Adjustment for a Cation Exchange Column Eluate Buffer

Assembly of Quorum Sensing Pathway Enzymes onto Patterned Microfabricated Devices

Abstract :-
This approach is based on electrodeposition of the aminopolysaccharide chitosan onto a selected electrode pattern of the device, and covalent conjugation of a target protein to chitosan upon biochemical activation of a genetically fused C-terminal pentatyrosine “pro-tag.” With this approach, assembly is “spatially selective”, occurring only at selected electrode patterns, and the entire process occurs under mild experimental conditions. Additionally, assembly is reversible and the devices reusable, as the deposited chitosan can be removed by simple incubation in dilute acid. Continue reading Assembly of Quorum Sensing Pathway Enzymes onto Patterned Microfabricated Devices

Application of Dynamic Light Scattering to Chemical and Biomolecular Engineering: Polymers, Proteins, and Liquid Crystals

Abstract :-
Dynamic Light Scattering (DLS) is a powerful tool for probing soft-matter. The history, equipment, and basic theory of DLS is outlined. Three applications of DLS representing contemporary problems in chemical and biomolecular engineering are presented: polymers, proteins, and liquid crystals. DLS was performed on the polymer poly(ethylene glycol) in aqueous solutions to discern a conformational change from a coil to a helix when dissolved in isobutyric acid and to compare DLS and small angle neutron scattering (SANS) measurements. Continue reading Application of Dynamic Light Scattering to Chemical and Biomolecular Engineering: Polymers, Proteins, and Liquid Crystals

Analysis of rheological properties and molecular weight distributions in continuous polymerization reactors

Abstract :-
This work explores the possibility of exploiting structure-property relationships to manufacture tailor-made polymers with target end-use properties. A novel framework which aims to improve upon current industrial practices in polymerization process and product quality control is proposed. The strong inter-relationship between the molecular architecture and rheological properties of polymers is the basis of this framework. The melt index is one of the most commonly used industrial measures of a polymer’s processibilty. However, this single-point non-Newtonian viscosity is inadequate to accurately reflect the polymer melt’s flow behavior. This justifies monitoring the entire viscosity-shear rate behavior during the polymerization stage. Continue reading Analysis of rheological properties and molecular weight distributions in continuous polymerization reactors

Analysis of Drug Delivery in the Eye Using Magnetic Resonance Imaging

Analysis of Drug Delivery in the Eye Using Magnetic Resonance Imaging

Abstract :-
With the rapid increase in the elderly population, the number of Americans afflicted with vision impairment due to ocular disease is projected to rise substantially by the year 2020. Ocular disorders are becoming a major public health problem, and efforts have increased in recent years to develop methods of efficient drug delivery. Currently, the most effective method for treating serious ocular disorders is to inject drug solutions directly into the vitreous. However, injecting in this manner carries a high risk of severe side effects. As a safer alternative, researchers in recent years have been investigating transscleral drug delivery, in which the drug is administered to the outer coat of the eye. Continue reading Analysis of Drug Delivery in the Eye Using Magnetic Resonance Imaging