Optimization of PCR Conditions to Amplify Microsatellite loci in the bunchgrass lizard (Sceloporus slevini) genomic DNA

Abstract :-
Microsatellites, also called Simple Sequence Repeats (SSRs), repetitions of nucleotide motifs of 1-5 bases, are currently the markers of choice due to their abundant distribution in the genomes, and suitability for high-throughput analysis. A total of five different primer pairs were optimized for polymerase chain reaction (PCR) to amplify microsatellite loci in total genomic DNA of bunchgrass lizards (Sceloporus slevini) collected from three sites in southeastern Arizona; the Sonoita Plain, Chiricahua Mountains and Huachuca Mountains.

he PCR conditions standardized for microsatellite amplification in this study were an initial denaturation at 94°C for five minutes; 35 cycles of denaturation (94°C) for 30 seconds, annealing (55°C) for 30 seconds, extension (72°C) for one minute followed by final extension at 72°C for four minutes.

Author:- Satya S Narina email, Christian A d’Orgeix email and Brian L Sayre email


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